Microarray analysis of replicative senescence
نویسندگان
چکیده
منابع مشابه
Microarray analysis of replicative senescence
BACKGROUND Limited replicative capacity is a defining characteristic of most normal human cells and culminates in senescence, an arrested state in which cells remain viable but display an altered pattern of gene and protein expression. To survey widely the alterations in gene expression, we have developed a DNA microarray analysis system that contains genes previously reported to be involved in...
متن کاملDifferential proteome analysis of replicative senescence in rat embryo fibroblasts.
Normal somatic cells undergo a finite number of divisions and then cease dividing whereas cancer cells are able to proliferate indefinitely. To identify the underlying mechanisms that limit the mitotic potential, a two-dimensional differential proteome analysis of replicative senescence in serially passaged rat embryo fibroblasts was undertaken. Triplicate independent two-dimensional gels conta...
متن کاملModeling of replicative senescence in hematopoietic development
Hematopoietic stem cells (HSC) give rise to an enormous number of blood cells throughout our life. In contrast their number of cell divisions preceding senescence is limited underin vitro culture conditions. Here we consider the question whether HSC can rejuvenate indefinitely or if the number of cell divisions is restricted. We have developed a multi-compartmental model for hematopoietic diffe...
متن کاملCSIG inhibits PTEN translation in replicative senescence.
Using a suppressive subtractive hybridization system, we identified CSIG (cellular senescence-inhibited gene protein; RSL1D1) that was abundant in young human diploid fibroblast cells but declined upon replicative senescence. Overexpression or knockdown of CSIG did not influence p21(Cip1) and p16(INK4a) expressions. Instead, CSIG negatively regulated PTEN and p27(Kip1) expressions, in turn prom...
متن کاملReplicative Senescence: An Old Lives' Tale?
underlying cause of organismic aging. This idea is much more controversial and less intuitively obvious. How did Life Sciences Division Department of Cancer Biology it come about, and what is the evidence for it? The observation that cell division is inherently limited contradicted an earlier belief, championed by Carrel, Normal animal cells, with few exceptions, do not divide that vertebrate c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Biology
سال: 1999
ISSN: 0960-9822
DOI: 10.1016/s0960-9822(99)80420-5